MRC National Institute for Medical Research, London
Science for Health
Martin Webb Publications
- Chisty, LT; Toseland, CP; Fili, N; Mashanov, GI; Dillingham, MS; Molloy, JE and Webb, MR (2013)
Monomeric PcrA helicase processively unwinds plasmid lengths of DNA in the presence of the initiator protein RepD.
Nucleic Acids Research [Epub ahead of print] PubMed abstract - Goncalves, MB; Dreyer, J; Lupieri, P; Barrera-Patino, C; Ippoliti, E; Webb, MR; Corrie, JET and Carloni, P (2013)
Structural prediction of a rhodamine-based biosensor and comparison with biophysical data.
Physical Chemistry Chemical Physics 15, 2177-2183 PubMed abstract - Webb, MR and Toseland, CP (2013)
Helicases.
In: Encyclopedia of Biophysics. Edited by Roberts, GCK.
Springer; EBSA, 2013. 950-958. - Arbore, C; Lewis, LM and Webb, MR (2012)
The kinetic mechanism of initiation by RepD as part of asymmetric, rolling circle plasmid unwinding.
Biochemistry 51, 3684-3693 PubMed abstract - Bianco, PR and Webb, MR (2012)
Helicase unwinding: active or merely perfect?
Journal of Molecular Biology 420, 139-140 PubMed abstract - Hedgethorne, K and Webb, MR (2012)
Fluorescent SSB as a reagentless biosensor for single-stranded DNA.
Methods in Molecular Biology 922, 219-233 PubMed abstract - Lallemand, P; Leban, N; Kunzelmann, S; Chaloin, L; Serpersu, EH; Webb, MR; Barman, T and Lionne, C (2012)
Transient kinetics of aminoglycoside phosphotransferase(3')-IIIa reveals a potential drug target in the antibiotic resistance mechanism.
FEBS Letters 586, 4223-4227 PubMed abstract - Toseland, CP; Powell, B and Webb, MR (2012)
ATPase cycle and DNA unwinding kinetics of RecG helicase.
PLOS ONE 7, e38270 PubMed abstract - Bickham, DC; West, TG; Webb, MR; Woledge, RC; Curtin, NA and Ferenczi, MA (2011)
Millisecond-scale biochemical response to change in strain.
Biophysical Journal 101, 2445-2454 PubMed abstract - Fili, N; Toseland, CP; Dillingham, MS; Webb, MR and Molloy, JE (2011)
A single-molecule approach to visualize the unwinding activity of DNA helicases.
Methods in Molecular Biology 778, 193-214 PubMed abstract - Kunzelmann, S and Webb, MR (2011)
Fluorescent detection of GDP in real time with the reagentless biosensor, rhodamine-ParM.
Biochemical Journal 440, 43-49 PubMed abstract - Toseland, CP and Webb, MR (2011)
Fluorescent nucleoside triphosphates for single-molecule enzymology.
Methods in Molecular Biology 778, 161-174 PubMed abstract - Yeeles, JTP; Gwynn, EJ; Webb, MR and Dillingham, MS (2011)
The AddAB helicase-nuclease catalyses rapid and processive DNA unwinding using a single Superfamily 1A motor domain.
Nucleic Acids Research 39, 2271-2285 PubMed abstract - Zhou, R; Kunzelmann, S; Webb, MR and Ha, T (2011)
Detecting intramolecular conformational dynamics of single molecules in short distance range with subnanometer sensitivity.
Nano letters 11, 5482-5488 PubMed abstract - Fili, N; Mashanov, GI; Toseland, CP; Batters, C; Wallace, MI; Yeeles, JTP; Dillingham, MS; Webb, MR and Molloy, JE (2010)
Visualizing helicases unwinding DNA at the single molecule level.
Nucleic Acids Research 38, 4448-4457 PubMed abstract - Ibanez-Garcia, D; Requejo-Isidro, J; Webb, MR; West, TG; French, P and Ferenczi, MA (2010)
Fluorescence lifetime imaging reveals that the environment of the ATP binding site of myosin in muscle senses force.
Biophysical Journal 99, 2163-2169 PubMed abstract - Kunzelmann, S; Morris, C; Chavda, AP; Eccleston, JF and Webb, MR (2010)
Mechanism of interaction between single-stranded DNA binding protein and DNA.
Biochemistry 49, 843-852 PubMed abstract - Kunzelmann, S and Webb, MR (2010)
A fluorescent, reagentless biosensor for ADP based on tetramethylrhodamine-labeled ParM.
ACS Chemical Biology 5, 415-425 PubMed abstract - Toseland, CP and Webb, MR (2010)
Fluorescence tools to measure helicase activity in real time.
Methods 51, 259-268 PubMed abstract - Webb, MR (2010)
Fluorescent biosensors to investigate helicase activity.
Methods in Molecular Biology 587, 13-27 PubMed abstract - Forgacs, E; Sakamoto, T; Cartwright, S; Belknap, B; Kovács, M; Tóth, J; Webb, MR; Sellers, JR and White, HD (2009)
The switch-1 mutation Ser217Ala converts myosinV into a low duty ratio motor.
Journal of Biological Chemistry 284, 2138-2149 PubMed abstract - Kunzelmann, S and Webb, MR (2009)
A biosensor for fluorescent determination of ADP with high time resolution.
Journal of Biological Chemistry 284, 33130-33138 PubMed abstract - Saikrishnan, K; Powell, B; Cook, NJ; Webb, MR and Wigley, DB (2009)
Mechanistic basis of 5'-3' translocation in SF1B helicases.
Cell 137, 849-859 PubMed abstract - Slatter, AF; Thomas, CD and Webb, MR (2009)
PcrA helicase tightly couples ATP hydrolysis to unwinding double-stranded DNA, modulated by the initiator protein for plasmid replication, RepD.
Biochemistry 48, 6326-6334 PubMed abstract - Toseland, CP; Martinez-Senac, MM; Slatter, AF and Webb, MR (2009)
The ATPase cycle of PcrA helicase and its coupling to translocation on DNA.
Journal of Molecular Biology 392, 1020-1032 PubMed abstract - West, TG; Hild, G; Siththanandan, VB; Webb, MR; Corrie, JET and Ferenczi, MA (2009)
Time course and strain dependence of ADP release during contraction of permeabilized skeletal muscle fibers.
Biophysical Journal 96, 3281-3294 PubMed abstract - Dillingham, MS; Tibbles, KL; Hunter, JL; Bell, JC; Kowalczykowski, SC and Webb, MR (2008)
A fluorescent single-stranded DNA binding protein as a probe for sensitive, real time assays of helicase activity.
Biophysical Journal 95, 3330-3339 PubMed abstract - Forgacs, E; Cartwright, S; Sakamoto, T; Sellers, JR; Corrie, JET; Webb, MR and White, HD (2008)
Kinetics of ADP dissociation from the trail and lead heads of actomyosinV following the power-stroke.
Journal of Biological Chemistry 283, 766-773 PubMed abstract - Sakamoto, T; Webb, MR; Forgacs, E; White, HD and Sellers, JR (2008)
Direct observation of the mechanochemical coupling in myosin Va during processive movement.
Nature 455, 128-132 PubMed abstract
[Page last updated 1 May 2013]
Webb group
Recent publications
Research projects
- DNA helicases: mechanism of coupling ATP hydrolysis to DNA translocation
- Myosins: the relationship between ATP hydrolysis and mechanical events
- Development of fluorescent biosensors and other probes
- The role of small G proteins in cell signalling
Expertise/techniques
Webb biography
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