MRC National Institute for Medical Research, London
Science for Health
Lyle Zimmerman group project:
High throughput reverse genetics/TILLING
We are collaborating with the Stemple group at the Sanger Institute and the Harland lab at UC Berkeley to create a Xenopus Mutation Resource for use in reverse genetic TILLING strategies. TILLING (Targeting Induced Local Lesions In Genomes) identifies carriers of mutations in specific sequences by amplifying and characterizing sequences from a large library of genomic DNAs harvested from individual mutagenized animals. Once a carrier of a mutation is identified, it can be recovered either from a cognate library of living animals or frozen sperm for phenotypic characterization.
Reverse genetics: TILLING mutant resource
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Targetting Induced Local Lesions In Genomes is a reverse genetic method based on sequencing amplicons(?) from mutagenized genomes for mutations in specific genes. Genomes bearing mutations in the gene of interest are recovered from a matching library of frozen sperm or living frogs.
Grants
A Xenopus tropicalis mutant resource, National Institutes of Health 1 R01 HD054354-01
Selected publications
- Abu-Daya, A; Sater, AK; Wells, DE; Mohun, TJ and Zimmerman, LB (2009)
Absence of heartbeat in the Xenopus tropicalis mutation muzak is caused by a nonsense mutation in cardiac myosin myh6.
Developmental Biology 336, 20-29 PubMed abstract - Khokha, MK; Krylov, V; Reilly, MJ; Gall, JG; Bhattacharya, D; Cheung, CYJ; Kaufman, S; Lam, DK; Macha, J; Ngo, C; Prakash, N; Schmidt, P; Tlapakova, T; Trivedi, T; Tumova, L; Abu-Daya, A; Geach, T; Vendrell, E; Ironfield, H; Sinzelle, L; Sater, AK; Wells, DE; Harland, RM and Zimmerman, LB (2009)
Rapid gynogenetic mapping of Xenopus tropicalis mutations to chromosomes.
Developmental Dynamics 238, 1398-1406 PubMed abstract - Goda, T; Abu-Daya, A; Carruthers, S; Clark, MD; Stemple, DL and Zimmerman, LB (2006)
Genetic screens for mutations affecting development of Xenopus tropicalis.
PLoS Genetics 2, 811-825 PubMed abstract
Zimmerman group
Recent publications
Research projects
- The frog Xenopus tropicalis
- Gynogenetic screens for chemically-induced mutations
- ENU phenotype database
- High throughput reverse genetics/TILLING
Resources
Gallery
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