MRC National Institute for Medical Research, London
Science for Health
High-throughput sequencing
High-throughput sequencing technologies are revolutionising molecular genetics, vastly expanding our ability to study genome structure, how genes are regulated and how cell and tissue differentiation occurs. Combined with increasingly sophisticated bioinformatic analysis, these methods of massively parallel sequencing-by-synthesis are likely to impact on all areas of basic biological research, with their ability to generate billions of bases of high-quality DNA sequence in a matter of days. In order to maintain its position at the forefront of basic research, NIMR has established a central next-generation sequencing facility.
This facility provides a cost-efficient service, producing rapid and highly accurate DNA and RNA sequence data for researchers at NIMR. By assisting at all stages from experimental design to data assembly and analysis, the service dramatically extends the ability of our scientists to make discoveries in genomics, epigenomics, gene expression analysis and protein-nucleic acid interactions. Establishment of the sequencing service coincides with the formation of the Division of Systems Biology and the appointment of two new research Programme Leaders.
High throughput sequencing data from a ChIP experiment in mouse
Major peaks show likely binding sites of the transcription factor under investigation, at different positions within a 144kb region around a known gene. Traces are derived from density of mapped sequence reads at 100 base intervals. Upper chart shows ChIP read density in blue and ‘input’ (control) read density in red; lower chart shows the difference between the ChIP and input read densities in green. Courtesy of Eva Kutejova
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