MRC National Institute for Medical Research, London
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Scientific interests
The lab is run by a senior scientific officer and a technician and supports one post-doctoral position. Our aim is to improve our imaging techniques, equipment and software by maintaining close collaborations with a number of projects which have unique imaging requirements. For example, we have active collaborations or have worked in the past to:
- Develop software for the quantification of the colocalisation of 2 or more fluorophores, with features not available in the commercial software packages.
- Analyse the segregation of cells in confocal data of drosophila embryos.
- Improve the accuracy of 3D datasets by studying the effects of noise and environmental parameters to the quality of the images.
- Automate the 3D reconstruction of the confocal images of serially sectioned transgenic mouse embryos.
- Develop a one-sided, out-of-focus light removal technique to improve the 3D reconstruction of serially sectioned mouse embryos.
- Track the movement of single cells in time-lapse microscope movies.
- Develop software to remove background autofluorescence from image datasets.
- Establish methods for fixing and mounting thick tissues for the preservation of their geometry under the microscope.
- Establish the correct choice of mounting medium for the minimization of optical errors which reduce resolution and laser penetration.
- Find methods for unbiased measurement of the number of cells forming the walls of vessels, from a small sample of images.
- Design a temperature controlled perfusion chamber for the long term imaging of living cells or perfused blood vessels.
Confocal microscopy imaging
- Techniques
- Equipment
- Scientific interests
- Future expansion
- Image gallery
- Recent publications
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