MRC National Institute for Medical Research, London
Science for Health
Digital image processing workshop
10 May 2010
Modern light microscopy is highly quantitative and involves collection, storage and analysis of digital images. The recent revolution in domestic digital imaging means that many people are now familiar with digital imaging and some will have experience of basic image processing and image manipulation.
A one-day Royal Microscopical Society workshop being held at NIMR will cover the underlying principles of digital image processing. The workshop will allow the advanced user of light microscopy to make the very best use of their imaging data. The types of image vary from those of high spatial resolution obtained using confocal microscopy to lower resolution video and multidimensional data obtained using advanced optical techniques (like FRET, FRAP, multiphoton).
This workshop course consists of two lectures that introduce the basic concepts of digital image processing and image analysis. The main focus of the day is for delegates to participate in a hands-on, computer-based workshop in which digital image analysis will be learnt by example. Workshop materials will be provided in advance of the meeting in the form of a CDROM and worksheets.
We cover many of the basic ideas underlying image processing such as filtering information in space, time and frequency domains and then we deal with image segmentation and image analysis whereby objects of interest are identified, counted, measured and tracked. The aim of this one-day course is to introduce the basics and get everyone to the point where they can start to experiment with image processing using public domain software like ImageJ and more sophisticated commercial packages
Justin Molloy
Workshop programme
| 09.00 | Tea/coffee | |
| 09.45 | Lecture - Justin Molloy, NIMR | An introduction to digital image processing and image analysis |
| 10.30 | Lecture - Peter Rosenthal, NIMR | The Fourier Transform |
| 10.45 | Computer Workshop | Data smoothing and signal processing |
| 12.30 | LUNCH | |
| 13.30 | Lecture - Paul Sheppard, Imaging Associates | The principles and methods of image processing and image analysis as applied to optical microscopy |
| 14.10 | Computer Workshop | Dealing with 2D and 3D datasets - more on smoothing, filtering and object recognition and rejection |
| 15.30 | Tea/coffee | |
| 15.50 | Workshop continues | Specialist techniques - image arithmetic, Fourier transform, kernals and convolution, B/W binary data, image statistics |
| 17.30 | FINISH |
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