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GMimPro

An image sequence processor for single molecule detection and tracking

This software is designed to analyse records containing images of single fluorescent molecules. It is provided free of charge for academic and non-profit making use. We ask that you please reference our publication (1) in Biophysical Journal if you use the programme in your published works.

GMimPro uses a special data format known as GMV. Every image in a sequence (record) has a header containing information about scales, time and so on. RAW data (image sequences) can be imported and converted into GMV file (File/Import data). Other file types (like TIFF) can be first converted into RAW data using 'ImageJ' or other software and converted into GMV. You can also download ImageJ plugins from below to read GMV files or convert other file types into GMV files.

Installation instructions

GMimPro is Windows software and will not run on Macs or Unix machines.

Download the compressed files and extract all files to any folder on your computer.

Note: The library files and the exe files must be in the same directory for the software to run.

Sample data files for use with GMimPro

About GMimPro

Algorithms used in GMimPro (described in detail in Mashanov and Molloy, 2007):

References:

  1. Mashanov, GI; Nobles, M; Harmer, SC; Molloy, JE and Tinker, A (2010)
    Direct observation of individual KCNQ1 potassium channels reveals their distinctive diffusive behaviour.
    Journal of Biological Chemistry 285, 3664-3675 PubMed abstract
  2. Hern, JA; Baig, AH; Mashanov, GI; Birdsall, B; Corrie, JET; Lazareno, S; Molloy, JE and Birdsall, NJM (2010)
    Formation and dissociation of M1 muscarinic receptor dimers seen by total internal reflection fluorescence imaging of single molecules.
    Proceedings of the National Academy of Sciences of the United States of America 107, 2693-2698 PubMed abstract
  3. Mashanov, GI and Molloy, JE (2007)
    Automatic detection of single fluorophores in live cells.
    Biophysical Journal 92, 2199-2211 PubMed abstract
  4. Mashanov, GI; Tacon, D; Peckham, M and Molloy, JE (2004)
    The spatial and temporal dynamics of pleckstrin homology domain binding at the plasma membrane measured by imaging single molecules in live mouse myoblasts.
    Journal of Biological Chemistry 279, 15274-15280 PubMed abstract

File listings for zip files

Library files in GMimProLib

Files in GMimPro

Files in ImageJ Plugins

Updates

GMimPro will be updated periodically, and the help system will be enhanced in the near future. Please check back from time to time to ensure that you are using the latest version. (The library files will not need updating.)

Contact

Dr Gregory Mashanov .(JavaScript must be enabled to view this email address)

Disclaimer

This work is the property of the author(s) and MRC, UK. You understand that the software has not been widely tested on different systems or computer platforms and you therefore use the software at your own risk.

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